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nonimmune goat serum cat#sl038  (Beijing Solarbio Science)


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    Beijing Solarbio Science nonimmune goat serum cat#sl038
    Nonimmune Goat Serum Cat#Sl038, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nonimmune+serum/goat+serum+sl038/pm37689223-145-6-10
    Average 90 stars, based on 1 article reviews
    nonimmune goat serum cat#sl038 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Bone marrow-derived mesenchymal stem cells overexpressing MiR-21 efficiently repair myocardial damage in rats.
    Article Snippet: Myocardial tissue slices from rats were fixed with 0.4% paraformaldehyde (Solarbio, Shanghai, China) for 5 min. Then the slices were washed, dried and incubated with endogenous peroxidase blocking solution (Solarbio, Shanghai, China) for 10 min. After the tissues were washed with PBS, they were incubated with nonimmune serum (Solarbio, Shanghai, China) for 10 min, followed by factor VIII polyclonal antibody (Solarbio, Shangai, China) for 1h and rinsed with PBS.

    Blocking Assay:

    Article Title: Bone marrow-derived mesenchymal stem cells overexpressing MiR-21 efficiently repair myocardial damage in rats.
    Article Snippet: Myocardial tissue slices from rats were fixed with 0.4% paraformaldehyde (Solarbio, Shanghai, China) for 5 min. Then the slices were washed, dried and incubated with endogenous peroxidase blocking solution (Solarbio, Shanghai, China) for 10 min. After the tissues were washed with PBS, they were incubated with nonimmune serum (Solarbio, Shanghai, China) for 10 min, followed by factor VIII polyclonal antibody (Solarbio, Shangai, China) for 1h and rinsed with PBS.



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    LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit <t>IgG</t> and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.
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    LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit IgG and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.

    Journal: American Journal of Cancer Research

    Article Title: REST-repressed lncRNA LINC01801 induces neuroendocrine differentiation in prostate cancer via transcriptional activation of autophagy

    doi:

    Figure Lengend Snippet: LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit IgG and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.

    Article Snippet: Antibodies were used in ChIP assays as follows: anti-REST (Millipore, 17-641) and nonimmune rabbit serum IgG (Alpha Diagnostic International).

    Techniques: ChIP-sequencing, Binding Assay, Amplification, Quantitative RT-PCR